Journal: Frontiers in Cell and Developmental Biology

Article Title: Localized sclerostin accumulation in osteocyte lacunar-canalicular system is associated with cortical bone microstructural alterations and bone fragility in db/db male mice

doi: 10.3389/fcell.2025.1562764

Figure Lengend Snippet: Reduced osteoblast and osteoclast activity in 20-week-old male db/db diabetic mice. H&E (A) and TRAP (B) staining show reduced activity of both osteoblasts and osteoclasts in db/db mice (n = 5 per group). (C) Serum analysis shows lower osteocalcin, TRACP 5b, and CTX-1 levels, and higher sclerostin levels in db/db mice (n = 4-5 per group). Data are presented as mean ± SD, analyzed by unpaired Student’s t-test. *p < 0.05, **p < 0.01.

Article Snippet: For recombinant sclerostin (SCL) treatment, IDG-SW3 cells were treated with 100 ng/mL recombinant mouse sclerostin (HY- P70717 , MCE, China) for 48 h following 28 days of osteogenic induction.

Techniques: Activity Assay, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Localized sclerostin accumulation in osteocyte lacunar-canalicular system is associated with cortical bone microstructural alterations and bone fragility in db/db male mice

doi: 10.3389/fcell.2025.1562764

Figure Lengend Snippet: Localized sclerostin accumulation and increased expression of PLR-related proteins in 20-week-old male db/db diabetic mice cortical bone. Immunohistochemical staining shows increased expression of CTSK (A) , MMP-13 (B) , and sclerostin (C) within the osteocyte LCS of db/db mice compared to WT mice. Linear regression analysis (D) reveals a significant positive correlation between sclerostin expression and CTSK and MMP-13 using combined data from both groups. Data are presented as mean ± SD. Statistical analysis was performed using an unpaired Student’s t-test for (A–C) (n = 5 mice per group) and Pearson correlation for (D) . *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: For recombinant sclerostin (SCL) treatment, IDG-SW3 cells were treated with 100 ng/mL recombinant mouse sclerostin (HY- P70717 , MCE, China) for 48 h following 28 days of osteogenic induction.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Localized sclerostin accumulation in osteocyte lacunar-canalicular system is associated with cortical bone microstructural alterations and bone fragility in db/db male mice

doi: 10.3389/fcell.2025.1562764

Figure Lengend Snippet: Effects of high glucose and recombinant Sclerostin (SCL) on osteocyte remodeling markers in IDG-SW3 cells. (A) Representative images showing osteocytic differentiation over 28 days, including DMP1-GFP fluorescence, ALP staining, and ARS staining. (B) Time-course expression of late osteocytic markers (FGF23, MEPE, Pdpn, Phex, and Sost) over 28 days, presented as relative fold induction. (C) Elevated mRNA expression of Sost, Ctsk, Mmp-13, and Atp6v0d2 in IDG-SW3 cells treated with 20 mM glucose compared to control cells. (D) Upregulation of Ctsk and Mmp-13 mRNA levels in IDG-SW3 cells treated with recombinant SCL compared to vehicle control. Data represent mean ± SD from three independent experiments (n = 3). Statistical significance is indicated (*p < 0.05, **p < 0.01, ****p < 0.0001).

Article Snippet: For recombinant sclerostin (SCL) treatment, IDG-SW3 cells were treated with 100 ng/mL recombinant mouse sclerostin (HY- P70717 , MCE, China) for 48 h following 28 days of osteogenic induction.

Techniques: Recombinant, Fluorescence, Staining, Expressing, Control

Journal: Journal of Biological Chemistry

Article Title: Sclerostin Is a Novel Secreted Osteoclast-derived Bone Morphogenetic Protein Antagonist with Unique Ligand Specificity

doi: 10.1074/jbc.m301716200

Figure Lengend Snippet: FIG. 1. Expression of sclerostin during mouse development. A, frontal section of E11 head. The only tissue expressing sclerostin mRNA is the endothelium of the pharyngeal artery (arrow). B, intense punc- tuated expression is seen at the sites of mandibular and maxillary bones at E15 (arrows). Meckel’s cartilage is negative. C, Bsp mRNA expression in osteoblasts marks the extent of bone formation at E16. D, scattered sclerostin mRNA-expressing cells are present on the surfaces of all bones in the head of a newborn mouse. The cells are abundant in the bone surrounding the growing tooth germs (arrows). E, in develop- ing long bones of E16 and E18 mouse embryos, sclerostin mRNA ex- pression is seen in the perichondrium and periosteum as well as in trabecular bone but not in the cartilage. White grains in dark field images indicate the expression of sclerostin mRNA. F, in a section through the ribs of E17 embryo, sclerostin mRNA is expressed in iso- lated large cells in the cartilage perichondrium. G, in the liver of E12 embryo, sclerostin mRNA expression is intense in hematopoietic cells. M, molar tooth germ; T, tongue; MC, Meckel’s cartilage; R, resting chondrocytes; P, proliferating chondrocytes; H, hypertrophic chondro- cytes; TB, trabecular bone; PC, perichondrium; PO, periosteum; RC, rib cartilage.

Article Snippet: After the cells had reached confluence, the medium was replaced with -minimum essential medium containing 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, and 50 g/ml ascorbic acid, and cells were cultured for 24 h. The cells were then cultured in -minimum essential medium containing 1% fetal bovine serum, 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, 50 g/ml ascorbic acid, 10 ng/ml recombinant human BMP2 (25 ng/ml), BMP4 (10 ng/ml), BMP6 (10 ng/ml), or BMP7 (25 ng/ml) protein (R&D Systems), either and 0–100 ng/ml recombinant mouse sclerostin protein or 100 ng/ml recombinant mouse noggin/Fc chimera (R&D Systems) for 72 h. Cells were washed twice with ice-cold PBS and scraped in 10 mM Tris-HCl-containing 2 mM MgCl2 and 0.05% Triton X-100, pH 8.2.

Techniques: Expressing

Journal: Journal of Biological Chemistry

Article Title: Sclerostin Is a Novel Secreted Osteoclast-derived Bone Morphogenetic Protein Antagonist with Unique Ligand Specificity

doi: 10.1074/jbc.m301716200

Figure Lengend Snippet: FIG. 2. Codistribution of sclerostin and MMP-9. The patterns of cells expressing sclerostin mRNA and MMP-9 (a marker of osteoclasts) mRNA are similar in the mandibular bone at E15 (A and B), in calvarial bone in the newborn mouse (NB) (C and D), and in the mandibular bone around the tooth germ in the newborn mouse (E and F).

Article Snippet: After the cells had reached confluence, the medium was replaced with -minimum essential medium containing 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, and 50 g/ml ascorbic acid, and cells were cultured for 24 h. The cells were then cultured in -minimum essential medium containing 1% fetal bovine serum, 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, 50 g/ml ascorbic acid, 10 ng/ml recombinant human BMP2 (25 ng/ml), BMP4 (10 ng/ml), BMP6 (10 ng/ml), or BMP7 (25 ng/ml) protein (R&D Systems), either and 0–100 ng/ml recombinant mouse sclerostin protein or 100 ng/ml recombinant mouse noggin/Fc chimera (R&D Systems) for 72 h. Cells were washed twice with ice-cold PBS and scraped in 10 mM Tris-HCl-containing 2 mM MgCl2 and 0.05% Triton X-100, pH 8.2.

Techniques: Expressing, Marker

Journal: Journal of Biological Chemistry

Article Title: Sclerostin Is a Novel Secreted Osteoclast-derived Bone Morphogenetic Protein Antagonist with Unique Ligand Specificity

doi: 10.1074/jbc.m301716200

Figure Lengend Snippet: FIG. 3. Detection of recombinant mouse sclerostin protein. A, the cell lysate and culture medium of cells expressing recombinant mouse sclerostin protein were separated by SDS-polyacrylamide gel electrophoresis under reducing (with 1,4-dithiothreitol; DTT) or non- reducing (without 1,4-dithiothreitol; DTT) conditions followed by Western blotting analysis with anti-E tag antibodies. B, purified recom- binant mouse sclerostin (0.35 g) was separated by SDS-polyacryl- amide gel electrophoresis under reducing conditions and subjected to protein staining.

Article Snippet: After the cells had reached confluence, the medium was replaced with -minimum essential medium containing 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, and 50 g/ml ascorbic acid, and cells were cultured for 24 h. The cells were then cultured in -minimum essential medium containing 1% fetal bovine serum, 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, 50 g/ml ascorbic acid, 10 ng/ml recombinant human BMP2 (25 ng/ml), BMP4 (10 ng/ml), BMP6 (10 ng/ml), or BMP7 (25 ng/ml) protein (R&D Systems), either and 0–100 ng/ml recombinant mouse sclerostin protein or 100 ng/ml recombinant mouse noggin/Fc chimera (R&D Systems) for 72 h. Cells were washed twice with ice-cold PBS and scraped in 10 mM Tris-HCl-containing 2 mM MgCl2 and 0.05% Triton X-100, pH 8.2.

Techniques: Recombinant, Expressing, Polyacrylamide Gel Electrophoresis, Western Blot, Purification, Nucleic Acid Electrophoresis, Staining

Journal: Journal of Biological Chemistry

Article Title: Sclerostin Is a Novel Secreted Osteoclast-derived Bone Morphogenetic Protein Antagonist with Unique Ligand Specificity

doi: 10.1074/jbc.m301716200

Figure Lengend Snippet: FIG. 4. Effects of sclerostin and nog- gin on alkaline phosphatase activity in MC3T3-E1 cells induced by BMPs. MC3T3-E1 cells were treated with BMP6 (10 ng/ml) (A), BMP7 (25 ng/ml) (B), BMP2 (25 ng/ml) (C), or BMP4 (10 ng/ml) (D) and different concentrations of mouse recombinant sclerostin or 100 ng/ml nog- gin for 72 h. After treatment, alkaline phosphatase activity in MC3T3-E1 cells was determined. Results are the means S.D. for five independent wells.

Article Snippet: After the cells had reached confluence, the medium was replaced with -minimum essential medium containing 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, and 50 g/ml ascorbic acid, and cells were cultured for 24 h. The cells were then cultured in -minimum essential medium containing 1% fetal bovine serum, 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, 50 g/ml ascorbic acid, 10 ng/ml recombinant human BMP2 (25 ng/ml), BMP4 (10 ng/ml), BMP6 (10 ng/ml), or BMP7 (25 ng/ml) protein (R&D Systems), either and 0–100 ng/ml recombinant mouse sclerostin protein or 100 ng/ml recombinant mouse noggin/Fc chimera (R&D Systems) for 72 h. Cells were washed twice with ice-cold PBS and scraped in 10 mM Tris-HCl-containing 2 mM MgCl2 and 0.05% Triton X-100, pH 8.2.

Techniques: Activity Assay, Recombinant

Journal: Journal of Biological Chemistry

Article Title: Sclerostin Is a Novel Secreted Osteoclast-derived Bone Morphogenetic Protein Antagonist with Unique Ligand Specificity

doi: 10.1074/jbc.m301716200

Figure Lengend Snippet: FIG. 5. Binding of sclerostin to BMP6. Mouse recombinant sclerostin was fixed on the carboxylmethyl sensor tip. The binding of different concentrations of BMP6 on the tip was analyzed using the BIAcore 2000 system.

Article Snippet: After the cells had reached confluence, the medium was replaced with -minimum essential medium containing 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, and 50 g/ml ascorbic acid, and cells were cultured for 24 h. The cells were then cultured in -minimum essential medium containing 1% fetal bovine serum, 100 units/ml penicillin G, 100 g/ml streptomycin, 10 mM -glycerophosphate, 50 g/ml ascorbic acid, 10 ng/ml recombinant human BMP2 (25 ng/ml), BMP4 (10 ng/ml), BMP6 (10 ng/ml), or BMP7 (25 ng/ml) protein (R&D Systems), either and 0–100 ng/ml recombinant mouse sclerostin protein or 100 ng/ml recombinant mouse noggin/Fc chimera (R&D Systems) for 72 h. Cells were washed twice with ice-cold PBS and scraped in 10 mM Tris-HCl-containing 2 mM MgCl2 and 0.05% Triton X-100, pH 8.2.

Techniques: Binding Assay, Recombinant

Journal: eLife

Article Title: Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

doi: 10.7554/eLife.64393

Figure Lengend Snippet: ( A ) FSS causes the rapid loss of sclerostin protein through a number of molecular mediators. ( B ) Ocy454 cells (n = 3–4) or ( C ) UMR106 cells (n = 3) were exposed to 1 min of FSS at 4 dynes/cm 2 and lysed 5 min post-flow. Western blots were probed for sclerostin, GAPDH, pCaMKII, and total CaMKII. ( D ) Sixteen week old female C57Bl/6 mice were ulnar loaded (1800 με, 90 s, 2 Hz), cortical osteocyte-enriched lysates isolated 5 min post-load, and western blots probed for sclerostin (n = 10 mice), pCaMKII, and total CaMKII (n = 5 mice). Sclerostin abundance relative to the loading control or pCaMKII relative to total CaMKII was quantified. ( E ) Ocy454 cells with endogenous sclerostin (n = 2), ( F ) UMR106 cells with endogenous sclerostin (n = 4), or ( G ) Ocy454 cells transfected with Myc-tagged sclerostin (n = 1) were subjected to 5 min of FSS at 4 dynes/cm 2 and lysed at the indicated times post-flow. Western blots were probed for sclerostin and β-actin. A representative time course is shown for each. Sclerostin abundance relative to the loading control was quantified. For each antibody, western blots are from a single gel and exposure; a vertical black line indicates removal of irrelevant lanes. Graphs depict mean ± SD. *p<0.05, **p<0.01 by unpaired two-tailed t-tests ( B–D ).

Article Snippet: Transfected construct ( Mus musculus ) , myc-tagged sclerostin , Origene , #MR222588 , .

Techniques: Western Blot, Isolation, Transfection, Two Tailed Test

Journal: eLife

Article Title: Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

doi: 10.7554/eLife.64393

Figure Lengend Snippet: ( A ) Seventeen week old male mice were subjected to a single bout of ulnar loading (1800 με), and ulnae were fixed in warm formalin 5 min after loading. Cryosections were stained for sclerostin to identify sclerostin-positive osteocytes, which were counted and presented as a proportion of total osteocytes in a selected ROI (n = 6). Yellow arrows indicate sclerostin-negative osteocytes, defined by the presence of DAPI staining but without detectable sclerostin. Yellow asterisks indicate non-specific staining. ( B ) Ocy454 cells transfected with GFP-sclerostin were subjected to 5 min of FSS at 4 dynes/cm 2 and lysed immediately post-flow. Western blots were probed for sclerostin and β-actin. ( C ) Ocy454 cells were subjected to 5 min of FSS at 4 dynes/cm 2 and lysed immediately post-flow. Western blots were probed for pro-collagen type 1α1 and β-actin. ( D, E ) Ocy454 cells were treated with PTH (10 nM) for 30 min and lysed. Western blots were probed for sclerostin, β-actin, pro-collagen type 1α1, and α-tubulin.

Article Snippet: Transfected construct ( Mus musculus ) , myc-tagged sclerostin , Origene , #MR222588 , .

Techniques: Staining, Transfection, Western Blot

Journal: eLife

Article Title: Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

doi: 10.7554/eLife.64393

Figure Lengend Snippet: ( A ) Ocy454 cells transfected with GFP-sclerostin were treated with vehicle or PTH (1–34) (10 nM) for the indicated time and were lysed. Western blots were probed for sclerostin and β-actin (n = 2–3). ( B ) Dissected tibiae flushed of marrow were treated with vehicle or PTH (1–34) (10 nM) for 30 min ex vivo and homogenized. Western blots were probed for sclerostin and β-actin (n = 6 mice). ( C ) Ocy454 cells were treated with vehicle or PTH (1–34) (10 nM) for the indicated time and were lysed. Western blots were probed for pCaMKII and total CaMKII (n = 6–8). Graphs depict mean ± SD. *p<0.05, **p<0.01 by two-way ANOVA with Holm–Sidak post hoc correction ( A, C ) or unpaired two-tailed t-test ( B ).

Article Snippet: Transfected construct ( Mus musculus ) , myc-tagged sclerostin , Origene , #MR222588 , .

Techniques: Transfection, Western Blot, Ex Vivo, Two Tailed Test

Journal: eLife

Article Title: Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

doi: 10.7554/eLife.64393

Figure Lengend Snippet: ( A ) Ocy454 cells transfected with GFP-sclerostin were treated with cycloheximide (150 µg/mL) to prevent new protein synthesis and either DMSO (0.1%), bafilomycin A1 (100 nM) to inhibit lysosomal degradation, brefeldin A (2 μm) to inhibit secretion, or MG-132 (10 μm) to inhibit the proteasome 4 hr prior to FSS. Cells were subjected to 5 min of FSS at 4 dynes/cm 2 and lysed immediately after the end of FSS or 30 min after the conclusion of FSS. Western blots were probed for sclerostin and β-actin. Time courses show mean ± SEM (n = 3–6 independent experiments/group). ( B ) Ocy454 cells transfected with GFP-sclerostin were pre-treated with DMSO (0.1%) or bafilomycin A1 (100 nM) to inhibit lysosomal degradation for 30 min prior to the addition of vehicle or PTH (1–34) (10 nM) for an additional 30 min (n = 3). Sclerostin abundance relative to the loading control was quantified. Graph depicts mean ± SD. *p<0.05, **p<0.01, ****p<0.0001 by two-way ANOVA with Holm–Sidak post hoc correction. ( C ) Amino acid sequences for sclerostin from mouse, human, rat, cow, and chicken were aligned using NCBI COBALT. Abbreviated sequences are shown and are annotated for putative lysosomal signal sequences. Full sequences are presented in .

Article Snippet: Transfected construct ( Mus musculus ) , myc-tagged sclerostin , Origene , #MR222588 , .

Techniques: Transfection, Western Blot

Journal: eLife

Article Title: Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

doi: 10.7554/eLife.64393

Figure Lengend Snippet: ( A ) Endogenous sclerostin (top) and GFP-tagged sclerostin (bottom) form discrete puncta in Ocy454 cells. ( B ) Ocy454 cells were transfected with GFP-sclerostin, and lysosomes were visualized with Lysotracker (1 mM, 1 hr) or siR-Lysosome (1 μM, 4 hr). Scale bar represents 10 μm. ( C ) Ocy454 cells were stained for endogenous sclerostin and either p62/sequestosome-1 or Rab27a to evaluate co-localization with these lysosome-associated proteins. ( D ) Ocy454 cells were exposed to 1 min of FSS at 4 dynes/cm 2 , lysed immediately post-flow, and western blotted for p62/sequestosome-1, β-actin, and LC3 (n = 4). ( E ) Ocy454 cells were treated with PTH (1–34) (10 nM) for 5 min, lysed, and western blotted for p62/sequestosome-1 and β-actin (n = 4) and LC3 (n = 8). ( F ) UMR106 cells were subjected to FSS for 5 min, then Magic Red Cathepsin B was applied for 10 min, fixed, and imaged to assess lysosome activity (n = 9). ( G ) Ocy454 cells were treated with DMSO or KN-93 (10 μM) to inhibit CaMKII for 1 hr prior to FSS at 4 dynes/cm 2 for 5 min before lysing immediately after FSS. Western blots were probed for p62/sequestosome-1 and β-actin (n = 3). ( H ) Ocy454 cells were transfected with a plasmid expressing either GFP or dominant negative CaMKII T286A prior to treatment with PTH (1–34) (10 nM) for 30 min. Western blots were probed for p62/sequestosome-1 and β-actin (n = 3). Graphs depict mean ± SD. *p<0.05, **p<0.01 by unpaired two-tailed t-test ( D–F ) or by two-way ANOVA with Holm–Sidak post hoc correction ( G, H ).

Article Snippet: Transfected construct ( Mus musculus ) , myc-tagged sclerostin , Origene , #MR222588 , .

Techniques: Transfection, Staining, Western Blot, Activity Assay, Plasmid Preparation, Expressing, Dominant Negative Mutation, Two Tailed Test

Journal: eLife

Article Title: Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

doi: 10.7554/eLife.64393

Figure Lengend Snippet: GFP-sclerostin-transfected and siR-lysosome-stained Ocy454 cells were imaged in Z-stacks with 0.5 μm steps and reconstructed into 3D images. Nikon general analysis was programmed to identify lysosomes containing sclerostin. GFP-sclerostin is pseudocolored in pink, siR-lysosome in yellow, and lysosomes containing sclerostin in cyan. Z-stack images from GFP-sclerostin- and siR-lysosome-labeled cells were analyzed for co-localization coefficients. M1 represents the overlap of sclerostin with siR-lysosome; M2 represents the overlap of siR-lysosome with sclerostin (n = 3).

Article Snippet: Transfected construct ( Mus musculus ) , myc-tagged sclerostin , Origene , #MR222588 , .

Techniques: Transfection, Staining, Labeling

Journal: eLife

Article Title: Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

doi: 10.7554/eLife.64393

Figure Lengend Snippet: ( A ) Ocy454 cells transfected with GFP-sclerostin were treated with vehicle (water) or 10 μM SNAP, a nitric oxide donor, and lysed after 5 min. Western blots were probed for sclerostin, α-tubulin, pCaMKII, and total CaMKII (n = 3). For each antibody, blots are from a single gel and exposure; a vertical black line indicates removal of irrelevant lanes. ( B ) Ocy454 cells transfected with myc-tagged sclerostin were treated with DMSO or bafilomycin A1 (100 nM) to inhibit lysosomal degradation, for 30 min, then treated with SNAP, a nitric oxide donor, for 5 min and lysed. Western blots were probed for sclerostin and α-tubulin (n = 2–3). ( C ) UMR106 cells were treated with vehicle or L-NAME (1 mM) to inhibit nitric oxide synthases (NOSs) for 1 hr and then exposed to 1 or 5 min of FSS. Lysates from cells exposed to 1 min of FSS were probed for p62/sequestosome-1 and α-tubulin abundance and lysates from cells exposed to 5 min of sclerostin were probed for sclerostin and α-tubulin abundance (n = 3). Graphs depict mean ± SD. *p<0.05, **p<0.01, ***p<0.001 by unpaired two-tailed t-test ( A ) or two-way ANOVA with Holm–Sidak post hoc test ( B , C ).

Article Snippet: Transfected construct ( Mus musculus ) , myc-tagged sclerostin , Origene , #MR222588 , .

Techniques: Transfection, Western Blot, Two Tailed Test

Journal: eLife

Article Title: Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

doi: 10.7554/eLife.64393

Figure Lengend Snippet: ( A ) UMR106 cells transfected with KillerRed imaged before and after stimulation with LED light. DCF was used to track ROS production. ( B ) UMR106 cells transfected with KillerRed were stimulated with LED light for 5 min and lysed 5 min after. Westerns were probed for sclerostin, GAPDH, pCaMKII, and total CaMKII.

Article Snippet: Transfected construct ( Mus musculus ) , myc-tagged sclerostin , Origene , #MR222588 , .

Techniques: Transfection

Journal: eLife

Article Title: Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

doi: 10.7554/eLife.64393

Figure Lengend Snippet:

Article Snippet: Transfected construct ( Mus musculus ) , myc-tagged sclerostin , Origene , #MR222588 , .

Techniques: Transfection, Construct, Sequencing, In Vitro, In Vivo, Software, Staining